RP titanium cap과 rhBMP-2를 이용하여 형성된 신생골의 미세전산화단층촬영을 이용한 평가
Evaluation of bone formation by recombinant human BMP-2 and rapid prototype titanium cap in rabbit calvaria using micro computed tomography
한만승, 정승곤, 김방신, 양지웅, 국민석, 박홍주, 유선열, 오희균,
소속 상세정보
한만승 ( Han Man-Seung ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
정승곤 ( Jung Seung-Gon ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
김방신 ( Kim Bang-Sin ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
양지웅 ( Yang Ji-Woong ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
국민석 ( Kook Min-Suk ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
박홍주 ( Park Hong-Ju ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
유선열 ( Ryu Sun-Youl ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
오희균 ( Oh Hee-Kyun ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
KMID : 0355620100360060466
Abstract
Introduction:This study examined the effect of recombinant human bone morphogenetic protein (rhBMP)-2 and β-tricalcium phosphate (β-TCP) on new bone formation in a rabbit calvarium using a rapid prototype titanium cap (RP Ti cap).
Materials and Methods : Eight New Zealand white rabbits were used in this study. Hemispherical RP Ti caps (10 mm in diameter) were implanted subperiosteally on the rabbit calvaria. β-TCP was filled in the RP Ti cap in the control group, and rhBMP-2 soaked β-TCP was used in experimental group. The rabbits were sacrificed 2 and 4 weeks after the operation. The volume and pattern of newly formed bone was analyzed by micro computed tomography (CT).
Results: Macroscopically, there were no abnormal findings in any of the animals. The micro CT images revealed new bone from the calvaria that expanded gradually toward the top of the titanium cap, particularly along the inner surface of the titanium cap in the experimental group at 4 weeks after grafting. There was no significant difference in new bone volume ratio between the control and experimental groups at 2 weeks after grafting. There was a statistically significant difference in the new bone volume ratio between the experimental (14.1±1.8 %) and control (7.2±1.5 %) groups at 4 weeks after grafting (P<0.01).
Conclusion: The RP Ti cap can effectively guide new bone formation and rhBMP-2 can induce the new bone formation.
키워드
Titanium cap; Bone morphogenetic protein 2; Micro computed tomography
원문 및 링크아웃 정보
등재저널 정보